On Soy Bean Urease: the Effect of Dilution, Acids, Alkalies and Ethyl Alcohol
نویسنده
چکیده
The conversion of urea into ammonium carbonate with the ureaser of the soy bean has proven of value not only in urea determinations but in cases where urea interferes with some chemical manipulation since its removal can be readily accomplished by this enzyme.2 Data relative to the action and behavior of urease under various conditions should prove of value in many connections.3 In our original paper on the use of urease in urea estimations, it was stated in discussing the time necessary for the complete decomposition of the urea in urine, that “The time required for the complete hydrolysis of the urea depends upon the quantity of urine used, the concentration of the urea, the amount of the enzyme present, and the temperature of the action. The velocity of the reaction is approximately twice as rapid at 35’ as at 25’ and directly proportional to the enzyme concentration within certain limits.” The experimental data for this statement are presented in part in the present communication. In order to determine the best conditions for the hydrolysis of urea by urease, the effect of various factors such as dilution, reaction of the medium, and enzyme concentration have been investigated.
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تاریخ انتشار 2003